研究成果

K. Uno, T. Sasaki, N. Sugimoto, H. Ito, T. Nishihara, S. Hagihara, T. Higashiyama, N. Sasaki, Y. Sato, K. Itami
Key Structural Elements of Unsymmetrical Cyanine Dyes for Highly Sensitive Fluorescence Turn-on DNA Probe.
Chem. Asian J. in press

Y. Kimata, T. Higaki, T. Kawashima, D. Kurihara, Y. Sato, T. Yamada, S. Hasezawa, F. Berger, T. Higashiyama, M. Ueda
Cytoskeleton dynamics control the first asymmetric cell division in Arabidopsis zygote.
Proc Natl Acad Sci USA, 113, 14157-14162. [PubMed]  [Article]

M. Takagi, T. Sakamoto, R. Suzuki, K. Nemoto, T. Obayashi, T. Hirakawa, TM. Matsunaga, D. Kurihara, Y. Nariai, T. Urano, T. Sawasaki, S. Matsunaga
Plant Aurora kinases interact with and phosphorylate transcription factors.
J. Plant Res., 129(6):1165-1178. [PubMed]  [Article]

M. Nambo, D. Kurihara, T. Yamada, T. Nishiwaki-Ohkawa, N. Kadofusa, Y. Kimata, K. Kuwata, M. Umeda, M. Ueda
Combination of Synthetic Chemistry and Live-Cell Imaging Identified a Rapid Cell Division Inhibitor in Tobacco and Arabidopsis thaliana.
Plant Cell Physiol., 57, 2255-2268. [PubMed]  [Article]

R.D. Kasahara, M. Notaguchi, S. Nagahara, T. Suzuki, D. Susaki, Y. Honma, D. Maruyama, T. Higashiyama 
Pollen tube contents initiate ovule enlargement and enhance seed coat development without fertilization.
Sci. Adv., 2:e1600554 [PubMed]  [Article]

E. Kozgunova, T. Higashiyama, D. Kurihara
Cytokinesis defect in BY-2 cells caused by ATP-competitive kinase inhibitors.
Plant Signal Behav., 11, e1238547. [PubMed]  [Article]

T. Suzuki, C. Matsushima, S. Nishimura, T. Higashiyama, M. Sasabe, Y. Machida
Identification of Phosphoinositide-Binding Protein PATELLIN2 as a Substrate of Arabidopsis MPK4 MAP Kinase during Septum Formation in Cytokinesis
Plant Cell Physiol. 57, 1744-1755 [PubMed]  [Article]

S. Fujii, T. Suzuki, P. Giegé, T. Higashiyama, N. Koizuka, T. Shikanai
The restorer-of-fertility-like 2 pentatricopeptide repeat protein and RNase P are required for the processing of mitochondrial orf291 RNA in Arabidopsis.
Plant J., 86, 504-513  [PubMed]  [Article]

A. Kanamori, Y. Sugita, Y. Yuasa, T. Suzuki, K. Kawamura, Y. Uno, K. Kamimura, Y. Matsuda, C.A. Wilson, A. Amores, J.H. Postlethwait, K. Suga, Y. Sakakura
A Genetic Map for the Only Self-Fertilizing Vertebrate.
G3 (Bethesda), 6, 1095-1106 [PubMed]  [Article] 

E. Kozgunova, T. Suzuki, M. Ito, T. Higashiyama, D.Kurihara
Haspin kinase has multiple functions in the plant cell division regulatory network.
Plant Cell Physiol., 57, 848-61 [PubMed]  [Article] 

A.G. Mizukami, R. Inatsugi, J. Jiao, T. Kotake, K. Kuwata, K. Ootani, S. Okuda, S.Sankaranarayanan, Y. Sato, D. Maruyama, H. Iwai, E. Garénaux, C. Sato, K. Kitajima, Y.Tsumuraya, H. Mori, J. Yamaguchi K, Itami., N. Sasaki, T. Higashiyama
The AMOR arabinogalactan sugar chain induces pollen-tube competency to respond to ovular guidance.
Curr. Biol., 26, 1091-1097 [PubMed]  [Article] 

A. Yoshida, S. Nakano, T. Suzuki, K. Ihara, T. Higashiyama, I. Mori
A glial K(+) /Cl(-) cotransporter modifies temperature-evoked dynamics in C. elegans sensory neurons.
Genes Brain Behav., 15, 429-440  [PubMed]  [Article]

Takeuchi H, Higashiyama T.
Tip-localized receptors control pollen tube growth and LURE sensing in Arabidopsis.
Nature 531, 245-248, doi: 10.1038/nature17413. [PubMed]  [Article] 

Ogasawara K, Yamada K, Hatsugai N, Imada C, Nishimura M.
Hexose Oxidase-Mediated Hydrogen Peroxide as a Mechanism for the Antibacterial Activity in the Red Seaweed Ptilophora subcostata.
PLoS One. 2016 Feb 11;11(2):e0149084.  doi: 10.1371/journal.pone.0149084.  [PubMed]

Kurihara D., Mizuta Y., Sato Y., Higashiyama T.
ClearSee: a rapid optical clearing reagent for whole-plant fluorescence imaging.
Development 142, 4168-4179. [PubMed]  [Article] 

Gooh K., Ueda M., Aruga K., Park J., Arata H., Higashiyama T., Kurihara D.
Live-cell imaging and optical manipulation of Arabidopsis early embryogenesis. 
Dev. Cell (2015) 34: 242-251. [PubMed]  [Article] 

Sasabe M, Ishibashi N, Haruta T, Minami A, Kurihara D, Higashiyama T, Nishihama R, Ito M, Machida Y.
The carboxyl-terminal tail of the stalk of Arabidopsis NACK1/HINKEL kinesin is required for its localization to the cell plate formation site.
J Plant Res., 128, 327-36  [PubMed]  [Article]

Michitaka Notaguchi, Tetsuya Higashiyama, Takamasa Suzuki
Identification of mRNAs That Move over Long Distances Using an RNA-Seq Analysis of Arabidopsis/Nicotiana benthamiana Heterografts.
Plant Cell Physiol., 56, 311-21,  [PubMed]  [PDF]

Mizuta Y, Kurihara D, Higashiyama T.
Two-photon imaging with longer wavelength excitation in intact Arabidopsis tissues
Protoplasma, in press.  [PubMed]  [Article]

Xian Jun Song, Takeshi Kuroha, Madoka Ayano, Tomoyuki Furuta, Keisuke Nagai, Norio Komeda, Shuhei Segami, Kotaro Miura, Daisuke Ogawa, Takumi Kamura, Takamasa Suzuki, Tetsuya Higashiyama, Masanori Yamasaki, Hitoshi Mori, Yoshiaki Inukai, Jianzhong Wu, Hidemi Kitano, Hitoshi Sakakibara, Steven E. Jacobsen, Motoyuki Ashikari
Rare Allele of A Novel Histone H4 Acetyltransferase Enhances Grain Weight, Yield and Plant Biomass in rice.
Proc. Natl. Acad. Sci. USA., 112, 76-81. [PubMed]  [Article]

Michitaka Notaguchi
Identification of phloem‑mobile mRNA
J Plant Res., 128, 27-35.  [PubMed]  [Article]

Yuki Hamamura, Moe Nishimaki, Hidenori Takeuchi, Anja Geitmann, Daisuke Kurihara & Tetsuya Higashiyama,
Live imaging of calcium spikes during double fertilization in Arabidopsis
Nature Commun. 5: 4722, 1-9 (2014)  [PubMed]  [Article]

Horade M, Yanagisawa N, Mizuta Y, Higashiyama T, Arata H
Growth assay of individual pollen tubes arrayed by microchannel device.
Microelectronic Engineering 118, 25-28.

Jongho Park, Daisuke Kurihara, Tetsuya Higashiyama, Hideyuki Arata
Fabrication of microcage arrays to fix plant ovules for long-term live imaging and observation.
Sensors & Actuators B: Chemical, 191, 178–185.  [Article]  [PDF]

Hida H., Nishiyama H., Sawa S., Higashiyama T., Arata H.
Behavior Analysis of Plant-parasitic Nematode in a Microchannel.
IEEE International Symposium on Micro-NanoMechatronics and Human Science 2013, pp. 142-145. (refereed proceeding paper)

Takeda S, Noguchi M, Hamamura Y, Higashiyama T.
Spatial distribution of the RABBIT EARS protein and effects of its ectopic expression in Arabidopsis thaliana flowers.
Planta, 2013 Dec 24. [Epub ahead of print]  [PubMed]  [Article]

Hida H., Nishiyama H., Sawa S., Higashiyama T., Arata H.
On-Chip Chemotaxis Assay of Plant-Parasitic Nematode Towards Increasing Global Crop Productivity.
MicroTAS 2013, pp. 1752-1754.

Yoko Arakaki, Hiroko Kawai-Toyooka, Yuki Hamamura, Tetsuya Higashiyama, Akira Noga, Masafumi Hirono, Bradley J. S. C. Olson, Hisayoshi Nozaki 
The Simplest Integrated Multicellular Organism Unveiled.
PLOS ONE, Dec. 11, 2013   [Article]

Mitsuhiro Horade, Masahiro M Kanaoka, Motoki Kuzuya, Tetsuya Higashiyama and Noritada Kaji
A microfluidic device for quantitative analysis　of chemoattraction in plants.
RSC Adv., 2013, Accepted Manuscript  [PDF]

Vu TM, Nakamura M, Calarco JP, Susaki D, Lim PQ, Kinoshita T, Higashiyama T, Martienssen RA, Berger F.
RNA-directed DNA methylation regulates parental genomic imprinting at several loci in Arabidopsis.
Development, 140, 2953-60.  [PubMed]  [Article]

Maruyama D, Hamamura Y, Takeuchi H, Susaki D, Nishimaki M, Kurihara D, Kasahara RD, Higashiyama T
Independent control by each female gamete prevents the attraction of multiple pollen tubes.
Dev Cell, 25, 317-323   [PubMed]  [Article]

Okuda S, Suzuki T, Mori H, Kanaoka M M, Sasaki N, Higashiyama T
Acquisition of LURE-Binding Activity at the Pollen Tube Tip of Torenia fournieri.
Mol. Plant, 6, 1074-1090   [PubMed]  [Article]

Kasahara D.R., Maruyama D., Higashiyama T.
Fertilization recovery system is dependent on the number of pollen grains for efficient reproduction in plants.
Plant Signal. Behav., vol.8(4), eLocation ID: e23690.  [PubMed]  [Article]
(his work adorn the cover of this issue)

Takeuchi H., Higashiyama T.
A species-specific cluster of defensin-like genes encodes diffusible pollen tube attractants in Arabidopsis.
PLoS Biol., 10(12): e1001449. [PubMed]  [Article]

Jullien E.P., Susaki D., Yelagandula R., Higashiyama T., Berger F.
DNA methylation dynamics during sexual reproduction in Arabidopsis thaliana.
Curr. Biol., 22, 1825-1830. [PubMed]  [Article]

Suetsugu N, Sato Y, Tsuboi H, Kasahara M, Imaizumi T, Kagawa T, Hiwatashi Y, Hasebe M, Wada M.
The KAC family of kinesin-like proteins is essential for the association of chloroplasts with the plasma membrane in land plants.
Plant Cell Physiol., 53, 1854-1865  [PubMed]  [Article]

Horade M., Mizuta Y., Kaji N., Higashiyama T., Arata H.
PLANT-ON-A-CHIP MICROFLUIDIC-SYSTEM FOR QUANTITATIVE ANALYSIS OF POLLEN TUBE GUIDANCE BY SIGNALING MOLECULE: TOWARDS CELL-TO-CELL COMMUNICATION STUDY.
MicroTAS 2012, Proc. pp. 1027-1029. (refereed proceeding of the conference)

Nakamichi N., Kiba T., Kamioka M., Suzuki T., Yamashino T., Higashiyama T., Sakakibara H., Mizuno T.
Transcriptional repressor PRR5 directly regulates clock-output pathways.
Proc. Natl. Acad. Sci. USA 109, 17123-17128.  [PubMed]  [Article]

Luo L., Ando S., Sasabe M., Machida C., Kurihara D., Higashiyama T., Machida Y.
Arabidopsis ASYMMETRIC LEAVES2 protein required for leaf morphogenesis consistently forms speckles during mitosis of tobacco BY-2 cells via signals in its specific sequence.
J. Plant Res., [PubMed]  [Advance Online Publication]

Hamamura Y., Nagahara S., Higashiyama T.
Double fertilization on the Move.
Curr. Opin. Plant Biol., 15, 70-77.  [PubMed]  [Article]

Takeuchi H., Higashiyama T.
Attraction of tip-growing pollen tubes by the female gemtophyte.
Curr. Opin. Plant Biol., 14, 614-621.  [PubMed]  [Article]

Ikeda Y., Kinoshita Y., Susaki D., Ikeda Y., Iwano M., Takayama S., Higashiyama T., Kakutani T., Kinoshita T.
HMG domain containing SSRP1 is required for DNA demethylation and genomic imprinting in Arabidopsis.
Developmental Cell, 21, 589-596.  [PubMed]  [Article]

Adachi S., Minamisawa K., Okushima Y., Inagaki S., Yoshiyama K., Kondou Y., Kaminuma E., Kawashima M., Toyoda T., Matsui M., Kurihara D., Matsunaga S., Umeda M.
Programmed induction of endoreduplication by DNA double-strand breaks in Arabidopsis.
Proc Natl Acad Sci U S A., Vol. 108, 10004-10009, 2011.  [PubMed]  [Article]

Kanaoka M.M., Kawano N., Matsubara Y., Susaki D., Okuda S., Sasaki N., Higashiyama T.
Identification and characterization of TcCRP1, a pollen tube attractant from Torenia concolor.
Annals of Botany, Vol. 108, 739-747, 2011.  [PubMed]  [Article]

Kawano N., Susaki D., Sasaki N., Higashiyama T. , Kanaoka M. M.
Isolation of gametophytic cells and identification of their cell-specific markers in Torenia fournieri, T. concolor and L. micrantha.
Cytologia, Vol. 76, 177-184, 2011.

Kurihara D., Matsunaga S., Omura T., Higashiyama T., Fukui K.
Identification and characterization of plant Haspin kinase as a histone H3 threonine kinase.
BMC Plant Biology, Vol. 11, 73, 2011.  [PubMed]  [Article]

Hamamura Y., Saito C., Awai C., Kurihara D., Miyawaki A., Nakagawa T., Kanaoka M.M., Sasaki N., Nakano A., Berger F., Higashiyama T.
Live-cell Imaging Reveals the Dynamics of Two Sperm Cells during Double Fertilization in Arabidopsis thaliana.
Current Biol., Vol. 21, 497-502, 2011.   [PubMed]  [Article]

Goto H., Okuda S., Mizukami A., Mori H., Sasaki N., Kurihara D., Higashiyama T.
Chemical visualization of an attractant peptide, LURE.
Plant Cell Physiol., Vol. 52, 49-58, 2010.   [PubMed]  [Article]

リバネス研究費リブセンス賞　(2014)
野田口理孝

Hideyuki Arata, Tetsuya Higashiyama
Poly(dimethylsiloxane)-based microdevices for studying plant reproduction (invited)
Biochem. Soc. Trans. vol. 42 (2), pp. 320-324 (2014).

Kurihara D., Hamamura Y., Higashiyama T.
Live-cell analysis of plant reproduction: Live-cell imaging, optical manipulation, and advanced microscopy technologies.
Dev. Growth Differ. 2013 Feb 26. doi: 10.1111/dgd.12040.  [PubMed]  [Article]

Kasahara D.R., Hamamura Y., Higashiyama T.
Plant fertilization captured by live cell imaging.
In "Sperm Cell Research in the 21st Century: Historical Discoveries to New Horizons." (Ed. by M Morisawa) Adthree Publishing Co.Ltd., 2012 Aug; pp.190-195.

笠原 竜四郎
受精回復システムの発見
Plant Morphology, 25, 83-88

新田 英之，東山 哲也
極微小デバイスを用いた生体分子・細胞・組織の顕微操作と解析
Plant Morphology, 25, 61-66. 

佐藤 良勝,  門田 明雄
微光束照射装置付き蛍光イメージングシステムにより観えた葉緑体アクチン繊維の動態
Plant Morphology, 24, 19-22.  [J-STAGE]  [Article]

東山哲也 (2012) 被子植物の繁栄を支える重複受精の瞬間を見る. 遊ぶ (生命誌年間号 vol.69-72), 146-152

東山哲也 (2012) 植物の生殖細胞と受精戦略. 領域融合レビュー, 1, e007
[Article]

筒井大貴、東山哲也 (2012) レーザー熱膨張式微量インジェクターを用いた試料導入. 目的別で選べる　遺伝子導入プロトコール（実験医学　別冊）, pp.158-166

筒井大貴、東山哲也（2012）　レーザー熱膨張式マイクロインジェクションとその応用.　Plant Morphology, 24, 33-36.

東山哲也（2011）　被子植物の繁栄を支える重複受精の瞬間を見る.  季刊「生命誌」, pp.71.

栗原大輔（2011）　細胞分裂期において分裂期キナーゼが制御する染色体動態. Plant Morphology, 23, 81-89.

日本植物形態学会第27回総会・大会　ポスター賞 (2015)
小笠原 希実，比留川 治子, 桧垣 匠，秋田 佳恵，馳澤 盛一郎，東山 哲也
「顕微鏡画像データ提示における3Dプリンタの活用」

第127回講演会日本育種学会優秀発表賞 (2015)
野田口理孝, 佐藤良勝, 東山哲也
（タバコ属を用いた異科接木の挑戦　—タバコは育種に使えるか？—）

ナイスステップな研究者2014 (2015)
東山哲也

第3回「名古屋大学石田賞」（2014）
新田英之
「マイクロ工学による細胞・生命分子の高感度極限計測」

日本植物学会第78回大会若手奨励賞　(2014)
武内秀憲

日本分析化学会第63年会奨励賞　(2014)
新田英之

The 2nd ITbM Reserch Award (2014)
H. Arata, N. Yanagisawa, A. Sato, S. Oishi, M. Kanaoka
(Investigating Cooperative Effects of Multiple Signaling Molecules in Pollen Tube Guidance)

読売テクノ・フォーラム第20回ゴールド・メダル賞 (2014)
東山哲也

リバネス研究費リブセンス賞　(2014)
野田口理孝

一般財団法人総合研究奨励会（東京大学大学院工学系研究科）　平成25年度　総合研究奨励賞
新田英之

「動植物に共通するアロ認証機構の解明」第8回領域会議　最優秀ポスター賞
水多　陽子
（2光子顕微鏡を用いた花粉管の受精競争と多精拒否のin vivoライブイメージング）

2013年度　一般財団法人 エヌエフ基金　研究開発奨励賞
新田英之

平成24年度 化学とマイクロ・ナノシステム学会　若手優秀賞
新田 英之

平成24年度　日本植物形態学会　平瀬賞
笠原 竜四郎