Publications

Original papers

2017

J. Jiao, AG. Mizukami, S. Sankaranarayanan, J. Yamguchi, K. Itami, T. Higashiyama
Structure-Activity Relation of AMOR Sugar Molecule that Activates Pollen-Tubes for Ovular Guidance.

Plant Physiol. in press

H. Tsutsui, T. Higashiyama
pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene knockout in Arabidopsis thaliana.

Plant Cell Physiol. in press

K. Uno, T. Sasaki, N. Sugimoto, H. Ito, T. Nishihara, S. Hagihara, T. Higashiyama, N. Sasaki, Y. Sato, K. Itami
Key Structural Elements of Unsymmetrical Cyanine Dyes for Highly Sensitive Fluorescence Turn-on DNA Probe.
Chem. Asian J. in press

2016

Y. Kimata, T. Higaki, T. Kawashima, D. Kurihara, Y. Sato, T. Yamada, S. Hasezawa, F. Berger, T. Higashiyama, M. Ueda
Cytoskeleton dynamics control the first asymmetric cell division in Arabidopsis zygote.
Proc Natl Acad Sci USA, 113, 14157-14162. [PubMed] [Article]

M. Takagi, T. Sakamoto, R. Suzuki, K. Nemoto, T. Obayashi, T. Hirakawa, TM. Matsunaga, D. Kurihara, Y. Nariai, T. Urano, T. Sawasaki, S. Matsunaga
Plant Aurora kinases interact with and phosphorylate transcription factors.
J. Plant Res., 129(6):1165-1178. [PubMed] [Article]

M. Nambo, D. Kurihara, T. Yamada, T. Nishiwaki-Ohkawa, N. Kadofusa, Y. Kimata, K. Kuwata, M. Umeda, M. Ueda
Combination of Synthetic Chemistry and Live-Cell Imaging Identified a Rapid Cell Division Inhibitor in Tobacco and Arabidopsis thaliana.
Plant Cell Physiol., 57, 2255-2268. [PubMed] [Article]

R.D. Kasahara, M. Notaguchi, S. Nagahara, T. Suzuki, D. Susaki, Y. Honma, D. Maruyama, T. Higashiyama
Pollen tube contents initiate ovule enlargement and enhance seed coat development without fertilization.
Sci. Adv., 2:e1600554 [PubMed] [Article]

E. Kozgunova, T. Higashiyama, D. Kurihara
Cytokinesis defect in BY-2 cells caused by ATP-competitive kinase inhibitors.
Plant Signal Behav., 11, e1238547. [PubMed] [Article]

T. Suzuki, C. Matsushima, S. Nishimura, T. Higashiyama, M. Sasabe, Y. Machida
Identification of Phosphoinositide-Binding Protein PATELLIN2 as a Substrate of Arabidopsis MPK4 MAP Kinase during Septum Formation in Cytokinesis
Plant Cell Physiol. 57, 1744-1755 [PubMed] [Article]

S. Fujii, T. Suzuki, P. Giegé, T. Higashiyama, N. Koizuka, T. Shikanai
The restorer-of-fertility-like 2 pentatricopeptide repeat protein and RNase P are required for the processing of mitochondrial orf291 RNA in Arabidopsis.
Plant J., 86, 504-513 [PubMed] [Article]

A. Kanamori, Y. Sugita, Y. Yuasa, T. Suzuki, K. Kawamura, Y. Uno, K. Kamimura, Y. Matsuda, C.A. Wilson, A. Amores, J.H. Postlethwait, K. Suga, Y. Sakakura
A Genetic Map for the Only Self-Fertilizing Vertebrate.
G3 (Bethesda), 6, 1095-1106 [PubMed] [Article]

E. Kozgunova, T. Suzuki, M. Ito, T. Higashiyama, D.Kurihara
Haspin kinase has multiple functions in the plant cell division regulatory network.
Plant Cell Physiol., 57, 848-61 [PubMed] [Article]

A.G. Mizukami, R. Inatsugi, J. Jiao, T. Kotake, K. Kuwata, K. Ootani, S. Okuda, S.Sankaranarayanan, Y. Sato, D. Maruyama, H. Iwai, E. Garénaux, C. Sato, K. Kitajima, Y.Tsumuraya, H. Mori, J. Yamaguchi K, Itami., N. Sasaki, T. Higashiyama
The AMOR arabinogalactan sugar chain induces pollen-tube competency to respond to ovular guidance.
Curr. Biol., 26, 1091-1097 [PubMed] [Article]

A. Yoshida, S. Nakano, T. Suzuki, K. Ihara, T. Higashiyama, I. Mori
A glial K(+) /Cl(-) cotransporter modifies temperature-evoked dynamics in C. elegans sensory neurons.
Genes Brain Behav., 15, 429-440 [PubMed] [Article]

Takeuchi H, Higashiyama T.
Tip-localized receptors control pollen tube growth and LURE sensing in Arabidopsis.
Nature 531, 245-248, doi: 10.1038/nature17413. [PubMed] [Article]

Ogasawara K, Yamada K, Hatsugai N, Imada C, Nishimura M.
Hexose Oxidase-Mediated Hydrogen Peroxide as a Mechanism for the Antibacterial Activity in the Red Seaweed Ptilophora subcostata.
PLoS One. 2016 Feb 11;11(2):e0149084. doi: 10.1371/journal.pone.0149084. [PubMed]

2015

Kurihara D., Mizuta Y., Sato Y., Higashiyama T.
ClearSee: a rapid optical clearing reagent for whole-plant fluorescence imaging.
Development 142, 4168-4179. [PubMed] [Article]

Gooh K., Ueda M., Aruga K., Park J., Arata H., Higashiyama T., Kurihara D.
Live-cell imaging and optical manipulation of Arabidopsis early embryogenesis.
Dev. Cell (2015) 34: 242-251. [PubMed] [Article]

Daisuke Maruyama, Ronny Völz, Hidenori Takeuchi, Toshiyuki Mori, Tomoko Igawa, Daisuke Kurihara, Tomokazu Kawashima, Minako Ueda, Masaki Ito, Masaaki Umeda, Shuh-ichi Nishikawa, Rita Groß-Hardt, Tetsuya Higashiyama

Rapid Elimination of the Persistent Synergid through a Cell Fusion Mechanism

Cell (2015), http://dx.doi.org/10.1016/j.cell.2015.03.018

Sasabe M, Ishibashi N, Haruta T, Minami A, Kurihara D, Higashiyama T, Nishihama R, Ito M, Machida Y.
The carboxyl-terminal tail of the stalk of Arabidopsis NACK1/HINKEL kinesin is required for its localization to the cell plate formation site.
J Plant Res., 128, 327-36 [PubMed] [Article]

Michitaka Notaguchi, Tetsuya Higashiyama, Takamasa Suzuki
Identification of mRNAs That Move over Long Distances Using an RNA-Seq Analysis of Arabidopsis/Nicotiana benthamiana Heterografts.
Plant Cell Physiol., 56, 311-21, [PubMed] [PDF]

Mizuta Y, Kurihara D, Higashiyama T.
Two-photon imaging with longer wavelength excitation in intact Arabidopsis tissues
Protoplasma, in press. [PubMed] [Article]

Xian Jun Song, Takeshi Kuroha, Madoka Ayano, Tomoyuki Furuta, Keisuke Nagai, Norio Komeda, Shuhei Segami, Kotaro Miura, Daisuke Ogawa, Takumi Kamura, Takamasa Suzuki, Tetsuya Higashiyama, Masanori Yamasaki, Hitoshi Mori, Yoshiaki Inukai, Jianzhong Wu, Hidemi Kitano, Hitoshi Sakakibara, Steven E. Jacobsen, Motoyuki Ashikari
Rare Allele of A Novel Histone H4 Acetyltransferase Enhances Grain Weight, Yield and Plant Biomass in rice.
Proc. Natl. Acad. Sci. USA., 112, 76-81. [PubMed] [Article]

Michitaka Notaguchi
Identification of phloem‑mobile mRNA
J Plant Res., 128, 27-35. [PubMed] [Article]

2014

Yuki Hamamura, Moe Nishimaki, Hidenori Takeuchi, Anja Geitmann, Daisuke Kurihara & Tetsuya Higashiyama
Live imaging of calcium spikes during double fertilization in Arabidopsis
Nature Commun. 5: 4722, 1-9 (2014) [PubMed] [Article]

Hida H., Matsumura M., Kanno I., Nishiyama H., Sawa S., Higashiyama T., Arata H.

High-Throughput Chemotaxis Assay of Plant-Parasitic Nematode Toward Green Agriculture

Proc. MicroTAS 2014, (refereed proceeding paper)　accepted.

Yoko Mizuta and Tetsuya Higashiyama

Antisense gene inhibition by phosphorothioate antisense oligonucleotide in Arabidopsis pollen tubes.

Plant J., 78, 516-526 [PubMed] [PDF]

Horade M, Yanagisawa N, Mizuta Y, Higashiyama T, Arata H
Growth assay of individual pollen tubes arrayed by microchannel device.
Microelectronic Engineering 118, 25-28.

Jongho Park, Daisuke Kurihara, Tetsuya Higashiyama, Hideyuki Arata
Fabrication of microcage arrays to fix plant ovules for long-term live imaging and observation.
Sensors & Actuators B: Chemical, 191, 178–185. [Article] [PDF]

2013

Hida H., Nishiyama H., Sawa S., Higashiyama T., Arata H.
On-Chip Chemotaxis Assay of Plant-Parasitic Nematode Towards Increasing Global Crop Productivity.
MicroTAS 2013, pp. 1752-1754. (refereed proceeding paper)

Takeda S, Noguchi M, Hamamura Y, Higashiyama T.
Spatial distribution of the RABBIT EARS protein and effects of its ectopic expression in Arabidopsis thaliana flowers.
Planta, 2013 Dec 24. [Epub ahead of print] [PubMed] [Article]

Hida H., Nishiyama H., Sawa S., Higashiyama T., Arata H.
On-Chip Chemotaxis Assay of Plant-Parasitic Nematode Towards Increasing Global Crop Productivity.
MicroTAS 2013, pp. 1752-1754.

Yoko Arakaki, Hiroko Kawai-Toyooka, Yuki Hamamura, Tetsuya Higashiyama, Akira Noga, Masafumi Hirono, Bradley J. S. C. Olson, Hisayoshi Nozaki
The Simplest Integrated Multicellular Organism Unveiled.
PLOS ONE, Dec. 11, 2013 [Article]

Mitsuhiro Horade, Masahiro M Kanaoka, Motoki Kuzuya, Tetsuya Higashiyama and Noritada Kaji
A microfluidic device for quantitative analysis　of chemoattraction in plants.
RSC Adv., 2013, Accepted Manuscript [PDF]

Vu TM, Nakamura M, Calarco JP, Susaki D, Lim PQ, Kinoshita T, Higashiyama T, Martienssen RA, Berger F.
RNA-directed DNA methylation regulates parental genomic imprinting at several loci in Arabidopsis.
Development, 140, 2953-60. [PubMed] [Article]

Maruyama D, Hamamura Y, Takeuchi H, Susaki D, Nishimaki M, Kurihara D, Kasahara RD, Higashiyama T
Independent control by each female gamete prevents the attraction of multiple pollen tubes.
Dev Cell, 25, 317-323 [PubMed] [Article]

Okuda S, Suzuki T, Mori H, Kanaoka M M, Sasaki N, Higashiyama T
Acquisition of LURE-Binding Activity at the Pollen Tube Tip of Torenia fournieri.
Mol. Plant, 6, 1074-1090 [PubMed] [Article]

Kasahara D.R., Maruyama D., Higashiyama T.
Fertilization recovery system is dependent on the number of pollen grains for efficient reproduction in plants.
Plant Signal. Behav., vol.8(4), eLocation ID: e23690. [PubMed] [Article]
(his work adorn the cover of this issue)

2012

Takeuchi H., Higashiyama T.
A species-specific cluster of defensin-like genes encodes diffusible pollen tube attractants in Arabidopsis.
PLoS Biol., 10(12): e1001449. [PubMed] [Article]

Jullien E.P., Susaki D., Yelagandula R., Higashiyama T., Berger F.

DNA methylation dynamics during sexual reproduction in Arabidopsis thaliana.

Curr. Biol., 22, 1825-1830. [PubMed] [Article]

Suetsugu N, Sato Y, Tsuboi H, Kasahara M, Imaizumi T, Kagawa T, Hiwatashi Y, Hasebe M, Wada M.
The KAC family of kinesin-like proteins is essential for the association of chloroplasts with the plasma membrane in land plants.
Plant Cell Physiol., 53, 1854-1865 [PubMed] [Article]

Horade M., Mizuta Y., Kaji N., Higashiyama T., Arata H.

PLANT-ON-A-CHIP MICROFLUIDIC-SYSTEM FOR QUANTITATIVE ANALYSIS OF POLLEN TUBE GUIDANCE BY SIGNALING MOLECULE: TOWARDS CELL-TO-CELL COMMUNICATION STUDY.

Proc. microTAS 2012, Oct., Nov. Okinawa, Japan, pp. 1027-1029.

Nakamichi N., Kiba T., Kamioka M., Suzuki T., Yamashino T., Higashiyama T., Sakakibara H., Mizuno T.
Transcriptional repressor PRR5 directly regulates clock-output pathways.
Proc. Natl. Acad. Sci. USA 109, 17123-17128. [PubMed] [Article]

Kasahara D.R., Maruyama D., Hamamura Y., SakakibaraT., Twell D., Higashiyama T.
Fertilization Recovery after Defective Sperm Cell Release in Arabidopsis.
Curr. Biol., 22, 1084-1089. [PubMed] [Article]

Luo L., Ando S., Sasabe M., Machida C., Kurihara D., Higashiyama T., Machida Y.
Arabidopsis ASYMMETRIC LEAVES2 protein required for leaf morphogenesis consistently forms speckles during mitosis of tobacco BY-2 cells via signals in its specific sequence.
J. Plant Res., [PubMed] [Advance Online Publication]

Hamamura Y., Nagahara S., Higashiyama T.
Double fertilization on the Move.
Curr. Opin. Plant Biol., 15, 70-77. [PubMed] [Article]

2011

Adachi S., Minamisawa K., Okushima Y., Inagaki S., Yoshiyama K., Kondou Y., Kaminuma E., Kawashima M., Toyoda T., Matsui M., Kurihara D., Matsunaga S., Umeda M.
Programmed induction of endoreduplication by DNA double-strand breaks in Arabidopsis.
Proc Natl Acad Sci U S A., (in press)

Kanaoka M.M., Kawano N., Matsubara Y., Susaki D., Okuda S., Sasaki N., Higashiyama T.
Identification and characterization of TcCRP1, a pollen tube attractant from Torenia concolor.
Annals of Botany, (in press)

Kawano N., Susaki D., Sasaki N., Higashiyama T. , Kanaoka M. M.
Isolation of gametophytic cells and identification of their cell-specific markers in Torenia fournieri, T. concolor and L. micrantha.
Cytologia, (in press)

Kurihara D., Matsunaga S., Omura T., Higashiyama T., Fukui K.
Identification and characterization of plant Haspin kinase as a histone H3 threonine kinase.
BMC Plant Biology, Vol. 11, 73, 2011. [PubMed] [Article]

Hamamura Y., Saito C., Awai C., Kurihara D., Miyawaki A., Nakagawa T., Kanaoka M.M., Sasaki N., Nakano A., Berger F., Higashiyama T.
Live-cell Imaging Reveals the Dynamics of Two Sperm Cells during Double Fertilization in Arabidopsis thaliana.
Current Biol., Vol. 21, 497-502, 2011. [PubMed] [Article]

2010

Goto H., Okuda S., Mizukami A., Mori H., Sasaki N., Kurihara D., Higashiyama T.
Chemical visualization of an attractant peptide, LURE.
Plant Cell Physiol., Vol. 52, 49-58, 2010. [PubMed] [Article]